Theresa May's chief of staff denied he was working behind the Prime Minister's back to force through a second referendum today.
Gavin Barwell insisted he backed the PM's view that a new vote would be divisive for the country and a betrayal of Leave voters in 2016.
It was claimed today Mr Barwell and Mrs May's deputy David Lidington have begun drawing up plans for a new vote to break the impasse over the deal.
Mr Lidington repeated his view given to the Commons this week that a new referendum would be 'divisive but could not guarantee to be decisive'.
Education Secretary Damian Hinds insisted the idea had not been discussed by the Cabinet today despite claims a 'gang of five' top ministers wants it to happen.
Meanwhile SNP leader Nicola Sturgeon insisted Remain must be on the ballot paper of any new vote - and claimed chaos over Mrs May's deal had fuelled the case for Scottish independence.
Mrs May was accused by leading Remain campaigners of trying to run down the clock in a last-ditch attempt to get the votes for her deal.
Both Ms Sturgeon and Tory rebel Jo Johnson insisted the showdown vote on the Brexit deal - sensationally delayed from Tuesday - should be rescheduled for this week before the Commons breaks up for Christmas.
The Mail on Sunday revealed today Mr Barwell - the Prime Minister's most powerful and influential adviser - is understood to have decided that plans should be drawn up for another public poll.
Meanwhile Mr Lidington has held a series of secret meetings with Labour MPs to build a 'coalition of the willing' to force a new EU vote.
But Mr Barwell tweeted to Tory MP Robert Halfon today: 'Happy to confirm I do not want a second referendum - both for the reason you give and because it would further divide the country when we should be trying to bring people back together.'
He added: 'Happy to confirm I am not planning a second referendum with political opponents (or anyone else to anticipate the next question).'
The PM's aide insisted he was instead taking the morning off to play football and then put up a Christmas tree with his family.
Mr Hinds used an appearance on Sky News' Sophy Ridge on Sunday to dismiss today's reports about a new referendum.
Asked about the prospect of a poll, he insisted: 'No. Government policy couldn't be clearer.
'We are here to act on the will of the British people clearly expressed in the referendum.'
Mr Johnson, who quit as a minister to vote against the deal, warned on Sky News: 'We can't waste any more time.'
He demanded Mrs May bring back the vote on the deal before the Commons rises for Christmas on Thursday.
Yesterday, the Prime Minister tried to distract attention from the splits in her Government by launching a blistering attack on Tony Blair, accusing him of undermining her negotiations by encouraging Brussels to think that the UK will opt for a second referendum if her deal falls.
Mrs May said: 'There are too many people who want to subvert the process for their own political interests rather than acting in the national interest.
'For Tony Blair to go to Brussels and seek to undermine our negotiations by advocating for a second referendum is an insult to the office he once held and the people he once served.
'We cannot, as he would, abdicate responsibility for this decision. Parliament has a democratic duty to deliver what the British people voted for. I remain determined to see that happen. I will not let the British people down.'
No 10 is primed this weekend for an attempt by Labour leader Jeremy Corbyn to try to capitalise on Mrs May's problems by calling a vote of no confidence in the Government before Christmas.
If a majority of the 650 MPs of all parties in the Commons backed the motion, it could lead to a General Election.
Aides' nerves were not calmed by a poll released last night giving Labour a three-point lead. The Populus poll put Labour on 40 per cent, the Conservatives on 37 per cent and the Liberal Democrats on seven per cent. The pivot towards a possible second referendum will enrage the pro-Brexit backbenchers who led last week's failed attempt to topple Mrs May.
The Prime Minister was forced to pull the meaningful Commons vote on her Brexit deal after party whips told her she was likely to lose by a large margin, prompting backbenchers, led by Jacob Rees-Mogg's hard-Brexit European Research Group (ERG), to force a vote of no confidence in her leadership of the Tory party. Mrs May won by 200 votes to 117.
The Prime Minister subsequently failed to persuade the EU to offer a compromise on the notorious Northern Ireland 'backstop', leaving aides to conclude that she is unlikely to win the Commons round to her deal. That leaves just three realistic options: a softer Norway-style Brexit, a No Deal exit or a second referendum.
With large sections of the Tory party adamantly opposed to Norway, and a majority of MPs set against No Deal, The Mail on Sunday understands that Mr Barwell is considering the second public poll.
Sources say that Mr Barwell has told the pro-Remain 'gang of five' Cabinet Ministers – Mr Lidington, Chancellor Philip Hammond, Work and Pensions Secretary Amber Rudd, Justice Secretary David Gauke and Business Secretary Greg Clarke –that a second vote is 'the only way out of this'.
But the idea is being fiercely opposed by pro-Brexit Cabinet ministers led by Home Secretary Sajid Javid, Commons leader Andrea Leadsom and International Development Secretary Penny Mordaunt, who argue that it would be greeted with fury – and possibly civil disorder – by people who had voted for Brexit two years ago. And it puts Mr Barwell at loggerheads with the Prime Minister, who told this newspaper last week that the 2016 referendum was the only 'people's vote' required.
However, Mrs May's political capital has been damaged by the size of the vote against her on Wednesday. One senior source said: 'The PM is just a hostage of the Cabinet and her feuding aides'.
Ms Rudd hinted heavily about her support for a second referendum yesterday when she said that MPs across the parties should try to 'forge a consensus' over Brexit.
She said: 'We need to find a plan that a majority in Parliament can support. We need to try something different. Something that people do in the real world all the time, but which seems so alien in our political culture – to engage with others and be willing to forge a consensus'.
Could we even agree the question?
Before a second referendum could take place, there would be a bitter row over how it would work.
Some in No 10 see a chance to bypass Parliament and get public support for Theresa May's deal by pitching it in a straight fight with No Deal.
But Remainers say there must be an option to stay in the EU on the ballot paper – a scenario we have imagined above – and would use the Commons to try force it.
However a three-way question would not produce a conclusive result and would probably fall foul of electoral law. Therefore there could even be multiple rounds of voting, like a French presidential, election that would see No Deal go head-to-head with Remain in round two, if Mrs May's deal was rejected in a first vote.
Ministers are divided over whether Brexit could be delayed or stopped without new legislation. Attorney General Geoffrey Cox told Cabinet a new Act would have to be passed, but secret Government legal advice says Mrs May could unilaterally halt our exit using Royal Prerogative.
Cabinet Office Minister Mr Lidington has led the secret efforts to drum up support for a second poll by meeting senior Labour figures such as former Europe Minister Chris Bryant. Other 'back channels' have been established between Tory former Education Secretary Nicky Morgan and Labour's Yvette Cooper and between Tory Anna Soubry and Labour leadership hopeful Chuka Ummuna.
But the discussions have revealed bitter differences over the wording of any referendum question, with No 10 indicating that it should be between Mrs May's deal or No Deal. Labour MPs have made clear that the price of their support would be including Remain as an option.
Downing Street has been warned that some members of the ERG would be prepared to vote with Jeremy Corbyn in a Commons confidence motion, or abstain, rather than allow a second referendum.
Last night, former Tory leader Iain Duncan Smith described Mr Lidington's manoeuvring as 'utter madness'.
He told The Mail on Sunday: 'I don't believe he has ever accepted we will leave. Doing deals with a Labour Party run by Jeremy Corbyn is literally madness, utter madness. All the polling says if there is a second referendum we will plunge at the next Election. We will not be re-elected if we do not deliver Brexit.'
Mr Duncan Smith said Mr Lidington's actions encouraged the EU to play hardball in negotiations in the expectation that MPs would call a second referendum rather than risk No Deal.
'They are making it very hard for the PM to negotiate,' he said. 'The EU just don't believe we are willing to walk away.'
Meanwhile, Brexit supporting Cabinet Ministers such as Ms Leadsom and Ms Mordaunt are pushing for a 'managed No Deal' option. It would involve the UK paying up to £20 billion for a 'standstill' two years in which Britain would mirror all EU rules to allow both sides to prepare for a hard exit.
Mrs May's allies accuse Mr Rees-Mogg's ERG of making a second referendum more likely by sabotaging the Withdrawal Agreement.
One Brexiteer Minister described the anger in Government over the abortive coup: 'They like to present them as Jesuitical true believers, but they are more like jihadists'.
The Populus poll, carried out for Best for Britain, a pro referendum group, found that while 74 per cent of Labour voters support a second referendum, a majority of Tory voters, 51 per cent, are against.
Yes, Juncker insulted her but May's real foes are Macron and Varadkar
Theresa May won plaudits over her confrontation with EU President Jean-Claude Juncker last week, but privately she is also seething with French leader Emmanuel Macron and Ireland's Leo Varadkar.
Mrs May holds the French President personally responsible for 'trashing' her Brexit deal after he threatened to use the controversial Northern Irish backstop to extract concessions from Britain over fishing.
His comments sparked uproar among MPs and were in direct contrast to the agreed 'script' to help Mrs May sell the Withdrawal Agreement to the Commons. No 10 believe that meant they 'lost control of the narrative', turning MPs against the deal. Aides have told Paris of their outrage.
May and Macron had a frosty private meeting on Friday morning in Brussels, just before a visibly furious Mrs May confronted Mr Juncker.
Relations with Mr Varadkar are also cold, with sources claiming Mrs May 'loathes' the Irish Taoiseach. The PM is said to have 'outsourced' communication with Dublin to her No 2, David Lidington, because the relationship is so chilly.